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Title An intact plastid genome is essential for the survival of colorless Euglena longa but not Euglena gracilis Author info Lucia Hadariová ... [et al.] Author Hadariová Lucia (30%)
Co-authors Vesteg Matej 1982- (30%) UMBFP09 - Katedra biológie a ekológie
Birčák Erik (5%)
Schwartzbach Steven D. (5%)
Krajčovič Juraj (30%)
Source document Current Genetics : Microorganisms and Organelles. Vol. 63, no. 2 (2017), pp. 331-341. - Heidelberg : Springer-Verlag, 2017 Keywords antibiotiká - antibiotics chloroplastová DNA - chloroplast DNA degradation Language English Country United States of America systematics 53 Annotation Euglena gracilis growth with antibacterial agents leads to bleaching, permanent plastid gene loss. Colorless Euglena (Astasia) longa resembles a bleached E. gracilis. To evaluate the role of bleaching in E. longa evolution, the effect of streptomycin, a plastid protein synthesis inhibitor, and ofloxacin, a plastid DNA gyrase inhibitor, on E. gracilis and E. longa growth and plastid DNA content were compared. E. gracilis growth was unaffected by streptomycin and ofloxacin. Quantitative PCR analyses revealed a time dependent loss of plastid genes in E. gracilis demonstrating that bleaching agents produce plastid gene deletions without affecting cell growth. Streptomycin and ofloxacin inhibited E. longa growth indicating that it requires plastid genes to survive. This suggests that evolutionary divergence of E. longa from E. gracilis was triggered by the loss of a cytoplasmic metabolic activity also occurring in the plastid. Plastid metabolism has become obligatory for E. longa cell growth. A process termed "intermittent bleaching", short term exposure to subsaturating concentrations of reversible bleaching agents followed by growth in the absence of a bleaching agent, is proposed as the molecular mechanism for E. longa plastid genome reduction. Various non-photosynthetic lineages could have independently arisen from their photosynthetic ancestors via a similar process. Public work category ADC No. of Archival Copy 39416 Repercussion category LEANDER, Brian S. - LAX, Gordon - KARNKOWSKA, Anna - SIMPSON, Alastair G.B. Euglenida. In Handbook of the protists : second edition, 2017. ISBN 978-331928149-0, pp. 1047-1088.
ZECCA, Giovanni - GRASSI, Fabrizio - TABIDZE, Vazha - PIPIA, Ia - KOTORASHVILI, Adam - KOTARIA, Nato - BERIDZE, Tengiz. Dates and rates in grape's plastomes : evolution in slow motion. In Current genetics. ISSN 0172-8083, 2020, vol. 66, no. 1, pp. 123-140.
QIN HUAN - GUO QINGQING - LIU CHENCHEN - LI FENGLAN - ZHANG HUA - CHU ZIHAN - WANG JIANGXIN - LEI ANPING. Occurrence and light response of residual plastid genes in a Euglena gracilis bleached mutant strain OflB2. In Journal of oceanology and limnology. ISSN 2096-5508, 2020, vol. 38, no. 6, pp. 1858-1866.
O’NEILL, Ellis. Using new techniques to study old favorites: a case study of Euglena. In Handbook of algal science, technology and medicine. [Amsterdam] : Elsevier, 2020. ISBN 978-0-12-818305-2, pp. 161-170.
LI, Chao - CHEN, Zhenfan - CHEN, Zixi - LEI, Anping - LIU, Qiong - WANG, Jiangxin. Euglena-based synthetic biology and cell factory. In Algal Biotechnology. Wallingford : CABI International, 2023. ISBN 978-180062194-7, pp. 61-76.
Catal.org. BB301 - Univerzitná knižnica Univerzity Mateja Bela v Banskej Bystrici Database xpca - PUBLIKAČNÁ ČINNOSŤ References PERIODIKÁ-Súborný záznam periodika 
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Title A small portion of plastid transcripts is polyadenylated in the flagellate Euglena gracilis Author info Kristína Záhonová ... [et al.] Author Záhonová Kristína (30%)
Co-authors Hadariová Lucia (10%)
Vacula Rostislav (10%)
Yurchenko Vyacheslav (10%)
Eliáš Marek (10%)
Krajčovič Juraj (10%)
Vesteg Matej 1982- (20%)
Source document FEBS Letters. Vol. 588, no. 5 (2014), pp. 783-788. - Hoboken : John Wiley & Sons, 2014 Keywords červenoočká - Euglenoids - Euglenida gény pre ribozomálne RNA - ribozomal RNA genes chloroplastová DNA - chloroplast DNA rastliny - plants Language English Country Netherlands systematics 577 Annotation Euglena gracilis possesses secondary plastids of green algal origin. In this study, E. gracilis expressed sequence tags (ESTs) derived from polyA-selected mRNA were searched and several ESTs corresponding to plastid genes were found. PCR experiments failed to detect SL sequence at the 5'-end of any of these transcripts, suggesting plastid origin of these polyadenylated molecules. Quantitative PCR experiments confirmed that polyadenylation of transcripts occurs in the Euglena plastids. Such transcripts have been previously observed in primary plastids of plants and algae as low-abundance intermediates of transcript degradation. Our results suggest that a similar mechanism exists in secondary plastids. (C) 2014 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved. URL Link do databázy WoS Public work category ADC No. of Archival Copy 37273 Repercussion category SMITH, David Roy - KEELING, Patrick J. Protists and the Wild, Wild West of gene expression: new frontiers, lawlessness, and misfits. In Annual review of microbiology. ISSN 0066-4227, 2016, vol. 70, pp. 161-178.
GERASIMOV, Evgeny S. - GASPARYAN, Anna A. - KAUROV, Iosif - TICHÝ, Boris - LOGACHEVA, Maria D. - KOLESNIKOV, Alexander A. - LUKEŠ, Julius - YURCHENKO, Vyacheslav - ZIMMER, Sara L. - FLEGONTOV, Pavel. Trypanosomatid mitochondrial RNA editing : dramatically complex transcript repertoires revealed with a dedicated mapping tool. In Nucleic acids research. ISSN 0305-1048, 2018, vol. 46, no. 2, pp. 765-781.
DORRELL, Richard G. Convergence in the RNA processing of fractured algal organelle genomes. In Journal of phycology. ISSN 0022-3646, 2020, vol. 56, no. 5, pp. 1121-1123.
THIEN, Vun Yee - RODRIGUES, Kenneth Francis - VOO, Christopher Lok Yung - WONG, Clemente Michael Vui Ling - YONG, Wilson Thau Lym. Comparative transcriptome profiling of kappaphycus alvarezii (Rhodophyta, Solieriaceae) in response to light of different wavelengths and carbon dioxide enrichment. In Plants (Basel). ISSN 2223-7747, 2021, vol. 10, no. 6, pp. 1-17.
ZAKHAROVA, Alexandra - TASHYZREVA, Daria - BUTENKO, Anzhelika. A neo-functionalized homolog of host transmembrane protein controls localization of bacterial endosymbionts in the trypanosomatid Novymonas esmeraldas. In Current biology. ISSN 0960-9822, 2023, vol. 33, no. 13, pp. 2690–2701.
Database xpca - PUBLIKAČNÁ ČINNOSŤ References PERIODIKÁ-Súborný záznam periodika unrecognised