Počet záznamov: 1
MALDI-TOF MS analysis as a useful tool for an identification of Legionella pneumophila, a facultatively pathogenic bacterium interacting with free-living amoebae: A case study from water supply system of hospitals in Bratislava (Slovakia)
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$a 10.1016/j.exppara.2017.12.002 $2 DOI 035 $a biblio/61454 $2 CREPC2 100 $a 20180320 2018 m y slo 03 ba 101 0-
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$a MALDI-TOF MS analysis as a useful tool for an identification of Legionella pneumophila, a facultatively pathogenic bacterium interacting with free-living amoebae: A case study from water supply system of hospitals in Bratislava (Slovakia) $f Katarína Trnková ... [et al.] 330 $a Legionellae, i.e. Legionella pneumophila, are human bacterial hydrophilic facultative pathogens causing pneumonia (Legionnaires' disease). Free-living amoebae (FLA) can serve as natural hosts and thus as reservoirs of many amoebae-resistant bacteria. An encysted amoeba can contribute to the resistance of intracellular L. pneumophila to various chemical and physical treatments. Humans can be infected by droplets containing bacteria from an environmental source or human-made devices such as shower heads, bathtubs, air-conditioning units or whirlpools. In this study, we were investigating the presence of FLA and L pneumophila in plumbing systems of healthcare facilities in Bratislava (Slovakia) by standard diagnostic methods, while the presence of L pneumophila was verified also by MALDI-TOF MS (matrix-assisted laser desorption/ionization time-of-flight mass spectrometry) analysis. The results showed the occurrence of L pneumophila and FLA in 62.26% and 66.4% of samples taken from four paediatric clinics, respectively. Both standard methods and MALDI-TOF MS showed comparable results and they can be successfully applied for the identification of L pneumophila strains in environmental samples. Our approach could be useful for further monitoring, prevention and decreasing risk of Legionella infection also in other hospitals. (C) 2017 Elsevier Inc. All rights reserved. 463 -1
$1 001 umb_un_cat*0288351 $1 200 1 $a Experimental parasitology $v Vol. 184, (2018), pp. 97-102 $1 210 $a Amsterdam $c Elsevier B.V. $d 2018 $1 011 $a 0014-4894 $1 011 $a 1090-2449 606 0-
$3 umb_un_auth*0266444 $a amoebae-resistant bacteria 606 0-
$3 umb_un_auth*0266445 $a Acantharnoeba 606 0-
$3 umb_un_auth*0266446 $a environmental samples 606 0-
$3 umb_un_auth*0000071 $a prevencia $X prevention 606 0-
$3 umb_un_auth*0266447 $a Legionellosis 606 0-
$3 umb_un_auth*0266448 $a water supply safety 615 $n 502/504 $a Environmentálna veda 675 $a 502/504 700 -1
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Počet záznamov: 1