Počet záznamov: 1
A possible role for short introns in the acquisition of stroma-targeting peptides in the flagellate Euglena gracilis
SYS 0236591 LBL ---naa--22--------450- 005 20240513125233.9 014 $a 000281469400002 $2 WOS CC. SCIE 014 $a 000281469400002 $2 CCC 014 $a 2-s2.0-77955905618 $2 SCOPUS 014 $a 20587589 $2 MEDLINE 014 $a 20587589 $2 PubMed 017 70
$a 10.1093/dnares/dsq015 $2 DOI 101 0-
$a eng 102 $a GB 200 1-
$a A possible role for short introns in the acquisition of stroma-targeting peptides in the flagellate Euglena gracilis $f Matej Vesteg ... [et al.] 330 $a The chloroplasts of Euglena gracilis bounded by three membranes arose via secondary endosymbiosis of a green alga in a heterotrophic euglenozoan host. Many genes were transferred from symbiont to the host nucleus. A subset of Euglena nuclear genes of predominately symbiont, but also host, or other origin have obtained complex presequences required for chloroplast targeting. This study has revealed the presence of short introns (41-93 bp) either in the second half of presequence-encoding regions or shortly downstream of them in nine nucleus-encoded E. gracilis genes for chloroplast proteins (Eno29, GapA, PetA, PetF, Pet), PsaF, PsbM, PsbO, and PsbW). In addition, the E. gracilis Pbgd gene contains two introns in the second half of presequence-encoding region and one at the border of presequence-mature peptide-encoding region. Ten of 12 introns present within presequence-encoding regions or shortly downstream of them identified in this study have typical eukaryotic GT/AG borders, are T-rich, 45-50 bp long, and pairwise sequence identities range from 27 to 61%. Thus single recombination events might have been mediated via these cis-spliced introns. A double crossing over between these cis-spliced introns and trans-spliced introns present in 5'-UTRs of Euglena nuclear genes is also likely to have occurred. Thus introns and exon-shuffling could have had an important role in the acquisition of chloroplast targeting signals in E. gracilis. The results are consistent with a late origin of photosynthetic euglenids. 463 -1
$1 200 1 $a DNA research $v Vol. 17, no. 4 (2010), pp. 223-231 $1 011 $a 1340-2838 $1 210 $a Oxford $c Oxford University Press $d 2010 606 0-
$3 umb_un_auth*0256254 $a fotosystémy $X photosystems 606 0-
$3 umb_un_auth*0126053 $a chloroplasty $X chloroplasts 606 0-
$3 umb_un_auth*0054686 $a proteíny $X proteins 606 0-
$3 umb_un_auth*0256269 $a trypanozóma spavičná $Y Trypanosoma brucei 606 0-
$3 umb_un_auth*0178658 $a genómy $X genomes 615 $n 577 $a Biochémia. Molekulárna biológia. Biofyzika 675 $a 577 700 -1
$3 umb_un_auth*0255964 $a Vesteg $b Matej $f 1982- $9 40 $4 070 701 -1
$3 umb_un_auth*0255978 $a Vacula $b Rostislav $4 070 $9 10 701 -1
$3 umb_un_auth*0255986 $a Steiner $b Jürgen M. $4 070 $9 5 701 -1
$3 umb_un_auth*0255987 $a Mateášiková $b Blanka $4 070 $9 5 701 -1
$3 umb_un_auth*0255988 $a Loeffelhardt $b Wolfgang $4 070 $9 10 701 -1
$3 umb_un_auth*0255989 $a Brejová $b Broňa $4 070 $9 5 701 -0
$3 umb_un_auth*0031532 $a Krajčovič $b Juraj $4 070 $9 25 856 9-
$u http://gateway.isiknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=Alerting&SrcApp=Alerting&DestApp=WOS&DestLinkType=FullRecord&UT=WOS:000281469400002 $a $T=Priamy link $W=_new $XDL=1 T85 $x existuji fulltexy
Počet záznamov: 1