Expression of nucleus-encoded genes for chloroplast proteins in the flagellate Euglena gracilis

Vesteg, Matej, 1982- Expression of nucleus-encoded genes for chloroplast proteins in the flagellate Euglena gracilis / Matej Vesteg ... [et al.]. -- Reverse transcription PCRs (RT-PCRs), real-time RT-PCRs and microarrays containing 50-mer oligonucleotides representing nucleus-encoded genes for chloroplast proteins from Euglena gracilis were used to compare light- and dark-grown wild-type mRNA levels to those of light- and dark-grown E. gracilis stable white mutant strains W(gm)ZOflL, W(3)BUL and W(10)BSmL. The analyses revealed no light-dependent regulation of mRNA levels. Moreover, the mRNA levels of most genes were unchanged in all white mutants in comparison with wild-type. These results suggest that mRNA levels of nucleus-encoded genes for chloroplast proteins in E. gracilis do not depend on either light or plastid function.

In The Journal of Eukaryotic Microbiology. -- S. l. : The International Society of Protistologists, 2009. -- ISSN 1066-5234. -- ISSN 1550-7408. -- Vol. 56, no. 2 (2009), pp. 159-166

1. červenoočká 2. genómy 3. proteíny 4. chloroplasty

I. Vacula, Rostislav
II. Burey, Suzanne
III. Loeffelhardt, Wolfgang
IV. Drahovská, Hana
V. Martin, William
VI. Krajčovič, Juraj
VII. The Journal of Eukaryotic Microbiology. -- Vol. 56, no. 2 (2009), pp. 159-166

577