Expression of nucleus-encoded genes for chloroplast proteins in the flagellate Euglena gracilis

Vesteg, Matej, 1982-

Expression of nucleus-encoded genes for chloroplast proteins in the flagellate Euglena gracilis / Matej Vesteg ... [et al.]. -- Reverse transcription PCRs (RT-PCRs), real-time RT-PCRs and microarrays containing 50-mer oligonucleotides representing nucleus-encoded genes for chloroplast proteins from Euglena gracilis were used to compare light- and dark-grown wild-type mRNA levels to those of light- and dark-grown E. gracilis stable white mutant strains W(gm)ZOflL, W(3)BUL and W(10)BSmL. The analyses revealed no light-dependent regulation of mRNA levels. Moreover, the mRNA levels of most genes were unchanged in all white mutants in comparison with wild-type. These results suggest that mRNA levels of nucleus-encoded genes for chloroplast proteins in E. gracilis do not depend on either light or plastid function.

In The Journal of Eukaryotic Microbiology. -- S. l. : The International Society of Protistologists, 2009. -- ISSN 1066-5234. -- ISSN 1550-7408. -- Vol. 56, no. 2 (2009), pp. 159-166