Počet záznamov: 1
An intact plastid genome is essential for the survival of colorless Euglena longa but not Euglena gracilis
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$a 10.1007/s00294-016-0641-z $2 DOI 100 $a 20170524 2017 m y slo 03 ba 101 0-
$a eng 102 $a US 200 1-
$a An intact plastid genome is essential for the survival of colorless Euglena longa but not Euglena gracilis $f Lucia Hadariová ... [et al.] 330 $a Euglena gracilis growth with antibacterial agents leads to bleaching, permanent plastid gene loss. Colorless Euglena (Astasia) longa resembles a bleached E. gracilis. To evaluate the role of bleaching in E. longa evolution, the effect of streptomycin, a plastid protein synthesis inhibitor, and ofloxacin, a plastid DNA gyrase inhibitor, on E. gracilis and E. longa growth and plastid DNA content were compared. E. gracilis growth was unaffected by streptomycin and ofloxacin. Quantitative PCR analyses revealed a time dependent loss of plastid genes in E. gracilis demonstrating that bleaching agents produce plastid gene deletions without affecting cell growth. Streptomycin and ofloxacin inhibited E. longa growth indicating that it requires plastid genes to survive. This suggests that evolutionary divergence of E. longa from E. gracilis was triggered by the loss of a cytoplasmic metabolic activity also occurring in the plastid. Plastid metabolism has become obligatory for E. longa cell growth. A process termed "intermittent bleaching", short term exposure to subsaturating concentrations of reversible bleaching agents followed by growth in the absence of a bleaching agent, is proposed as the molecular mechanism for E. longa plastid genome reduction. Various non-photosynthetic lineages could have independently arisen from their photosynthetic ancestors via a similar process. 463 -1
$1 001 umb_un_cat*0293204 $1 011 $a 0172-8083 $1 011 $a 1432-0983 $1 200 1 $a Current Genetics $e Microorganisms and Organelles $v Vol. 63, no. 2 (2017), pp. 331-341 $1 210 $a Heidelberg $c Springer-Verlag $d 2017 606 0-
$3 umb_un_auth*0041201 $a antibiotiká $X antibiotics 606 0-
$3 umb_un_auth*0256264 $a chloroplastová DNA $X chloroplast DNA 606 0-
$3 umb_un_auth*0190063 $a degradation 615 $n 53 $a Chémia 675 $a 53 700 -1
$3 umb_un_auth*0255977 $a Hadariová $b Lucia $9 30 $4 070 701 -1
$3 umb_un_auth*0255964 $a Vesteg $b Matej $p UMBFP09 $4 070 $9 30 $f 1982- $T Katedra biológie a ekológie 701 -1
$3 umb_un_auth*0259469 $a Birčák $b Erik $4 070 $9 5 701 -1
$3 umb_un_auth*0255965 $a Schwartzbach $b Steven D. $4 070 $9 5 701 -0
$3 umb_un_auth*0031532 $a Krajčovič $b Juraj $4 070 $9 30 801 -0
$a SK $b BB301 $g AACR2 $9 unimarc sk T85 $x existuji fulltexy
Počet záznamov: 1